NeatCell
Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.
NeatCell is a protocol designed for isolation by means of a density gradient of the mononuclear cell (MNC) fraction from various cellular products into a small final volume of 8-20 mL. Alternatively, a fixed final volume of 45 mL can be chosen. In both cases, optimal granulocyte and Red Blood Cells (RBC) depletion is achieved.
Key features
| NeatCell features | |
| Initial volume | 30 to 120 mL |
| Selectable final volume | 8 to 20 mL, or 45 mL |
| Processing time | ~1h15 minutes |
| MNC recovery | > 52% |
| RBC depletion | > 99% |
Workflow
| Step | Details |
| 1 | Prepare a kit with density gradient medium (via microbial filter), washing solution, and a syringe for collecting the final product (if applicable). |
| 2 | Connect initial product to the kit. |
| 3 | Install the kit on the Sepax. |
| 4 | The Sepax automatically performs the procedure including: • Priming • Isolation via a density gradient medium • Washing |
| 5 | Collect purified mononuclear cells from the final product bag or via a syringe. |
Product features
- Automated procedure comprising of all required steps.
- Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
- Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
- Main user interface available in: Chinese, English, French, German, Italian, and Spanish.
Accessory for small final volume option
Use the separation chamber holder for the last steps of the procedure when collecting the final cells in a pre-connected syringe.
Kit / protocol compatibility matrix
| Kit / protocol | CS-490.1 | CS-470.1 | CS-900.2 | CS-430.1* |
| PeriCell | x | – | – | x |
| SmartRedux | x | – | – | – |
| ReadyCell | – | x | – | – |
| NeatCell | – | – | x | – |
| Adipose | – | – | x | – |
*Maximum initial volume limited to 600 mL.
Ordering information
NeatCell: Product #14300
Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.