CultureWash
Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.
CultureWash is designed for washing, exchanging medium, and concentrating cells. It offers flexible parameters and small final volume capabilities when manufacturing cellular therapy products.
![no-image](https://products.biosafe.ch/images/protocols/datasheet_culturewash.png)
Key features
Features | CultureWash |
Initial volume | 30 to 880 mL |
Selectable final volume | 8 to 220 mL* |
Average CD34+ recovery | >80% |
Average CD34+ viability | 95% |
*If product volume reduced to less than 20 mL, CS-900.2 single-use kit and Separation Chamber Holder are needed.
Workflow
Step | Details |
1 | Prepare a washing solution. |
2 | Prepare a kit with the initial product. |
3 | Install the kit on the Sepax. |
4 | The Sepax automatically performs the procedure including: • Primary dilution • Osmolarity balancing • Sedimentation and initial product rinsing • Supernatant extraction • Cell resuspension • Cell extraction into a final product bag. |
Product features
- Dilution ratio: Defines the volume of washing solution used to dilute the initial product for osmolarity balancing.
- Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
- Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
- Main user interface available in: English, French, German, Italian, and Spanish.
Kit / protocol compatibility matrix
Kit / protocol | CS-600.1 | CS-900.2** | CS-490.1 |
CordWash | x | – | – |
SmartWash | x | – | – |
CultureWash | x | x | – |
Dilution | x | x | x |
**The CS-900.2 kit is only validated for initial volumes up to 220 mL. Higher volumes must be validated by the user.
Ordering information
CultureWash: Product #14309
Separation Chamber Holder: Product #6033
Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.