Download PDFProduct #14200

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

UCB-HES is a protocol designed for concentrating the nucleated cell fraction from Umbilical Cord Blood (UCB) into a selectable final volume of 20-50 mL using Hydroxyethyl Starch (HES) sedimentation agent.

The sedimentation profile of the UCB-HES protocol has been designed to deliver high performances for a wide range of initial product characteristics, with reproducible final volumes.

Key features

UCB-HES features
Initial volume	35 to 320 mL
Selectable final volume	20 to 50 mL
Processing time	~ 35 minutes
Average TNC recovery	85 to 99 %
Average CD34+ recovery	90 to 99%

Workflow

Step	Details
1	Prepare a kit with the initial product.
2	Install the kit on the Sepax.
3	The Sepax automatically performs the procedure.
3	Collect the final product.

Product features

• Use both mono stopcock (reduced) or three stopcock (standard) kits.
• Separation profile customized to each cords’ unique characteristics.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: Chinese, English, French, German, Italian, and Spanish.

Kit / protocol compatibility matrix

Kit / protocol	CS-530.1	CS-530.4/.4b	CS-540.4/.4b	CS-570.4	CS-570.1m	CS-570.4mb	CS-570.3m	CS-430.1
UCB	x	x	x	x	x	x	x	x
UCB-HES	x	x	x	x	x	x	x	x

Ordering information

UCB-HES: Product #14200

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.

 Download PDFProduct #14201

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

UCB is a protocol designed for concentrating the nucleated cell fraction from Umbilical Cord Blood (UCB) into a selectable final volume of 10-50 mL without the use of a sedimentation agent.

The sedimentation profile of the UCB protocol has been designed to deliver high performances for a wide range of initial product characteristics, with reproducible final volumes.

Key features

UCB features
Initial volume	35 to 290 mL
Selectable final volume	10 to 50 mL
Processing time	~ 45 minutes
Average TNC recovery	80% – 99%
Average CD34+ recovery	85% – 99% (depending on initial cell count)

Workflow

Step	Details
1	Prepare a kit with the initial product.
2	Install the kit on the Sepax.
3	The Sepax automatically performs the procedure.
3	Collect the final product.

Product features

• Use both mono stopcock (reduced) or three stopcock (standard) kits.
• Separation profile customized to each cords’ unique characteristics.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: Chinese, English, French, German, Italian, and Spanish.

Kit / protocol compatibility matrix

Kit / protocol	CS-530.1	CS-530.4/.4b	CS-540.4/.4b	CS-570.4	CS-570.1m	CS-570.4mb	CS-570.3m	CS-430.1
UCB	x	x	x	x	x	x	x	x
UCB-HES	x	x	x	x	x	x	x	x

Ordering information

UCB: Product #14201

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.

 Download PDFProduct #14301

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

SmartRedux is a protocol designed for concentrating the nucleated cell fraction from various cellular products, processing an initial volume up to 3300 mL.

The SmartRedux protocol is particularly suitable for bone marrow volume reduction, due to the wide range of initial and final volumes.

Key features

SmartRedux features
Initial volume	30 to 3300 mL
Selectable final volume	up to 1500 mL
Average TNC recovery	> 75% (linked to applied concentration ratio)

Workflow

Step	Details
1	After bone marrow aspiration and filtration, connect initial product to the kit.
2	Install the kit on the Sepax.
3	The Sepax automatically performs the procedure.
4	Collect the concentrated cells from the final product bag.

Product features

• Fixed or proportional option available for the final volume, to fit different needs.
• Additional plasma parameter to optimize final buffy coat characteristics.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: English, French, German, Italian, and Spanish.

Kit / protocol compatibility matrix

Kit / protocol	CS-490.1	CS-470.1	CS-900.2	CS-430.1*
PeriCell	x	–	–	x
SmartRedux	x	–	–	–
ReadyCell	–	x	–	–
NeatCell	–	–	x	–
Adipose	–	–	x	–

*Maximum initial volume limited to 600 mL.

Ordering information

SmartRedux: Product #14301

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.

 Download PDFProduct #14302

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

ReadyCell is a protocol designed for concentrating the nucleated cell fraction from various cellular products into a small final volumes of 7-20 mL. It is particularly suitable for bone marrow concentration in point of care scenarios.

Key features

ReadyCell features
Initial volume	30 to 220 mL
Selectable final volume	7 to 20 mL
Processing time	~ 15 minutes
TNC concentration factor	Between 3 and 10

Workflow

Step	Details
1	After bone marrow aspiration and filtration, connect initial product to the kit.
2	Install the kit on the Sepax.
3	The Sepax automatically performs the procedure.
4	Collect concentrated cells from the final product vial.

Product features

• Simplified parameters and user actions for time-saving procedures.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: English, French, German, Italian, and Spanish.

Kit / protocol compatibility matrix

Kit / protocol	CS-490.1	CS-470.1	CS-900.2	CS-430.1*
PeriCell	x	–	–	x
SmartRedux	x	–	–	–
ReadyCell	–	x	–	–
NeatCell	–	–	x	–
Adipose	–	–	x	–

*Maximum initial volume limited to 600 mL.

Ordering information

ReadyCell: Product #14302

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.

 Download PDFProduct #14205

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

PlasmaFree is a protocol designed for routine processing of umbilical cord blood (UCB) to remove only plasma while conserving the nucleated cell component (buffy coat) fraction enriched in hematopoietic stem cells together with the red blood cells. This protocol does not require the addition of any sedimentation agent.

Key features

PlasmaFree features
Initial volume	35 to 440 mL
Selectable final volume	Flexible strategies available
Processing time	~ 20 minutes
Average TNC recovery	90 to 99 % (dependent on initial cell count)

Workflow

Step	Details
1	Prepare a kit with the initial product.
2	Install the kit on the Sepax.
3	The Sepax automatically performs the procedure.
3	Collect the final product.

Product features

• User selectable multiple final volume strategies.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: English, French, German, Italian, and Spanish.

Kit / protocol compatibility matrix

Kit / protocol	CS-570.4	CS-570.1m	CS-570.4mb	CS-570.3m	CS-430.1	CS-470.0
FamCord	x	x	x	x	–	–
PlasmaFree	–	–	–	–	x	x

Ordering information

PlasmaFree: Product #14205

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.

 Download PDFProduct #14203

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

PeriCell is a protocol designed for plasma depletion of apheresis products through a rapid procedure into a selectable final volume of 30-600 mL.

Key features

PeriCell features
Initial volume	100 to 840 mL
Selectable final volume	30 to 600 mL
Processing time	~ 20 minutes
Average TNC recovery	> 90%

Workflow

Step	Details
1	Prepare a kit with initial product.
2	Install the kit on the Sepax.
3	The Sepax automatically performs the procedure.
3	Collect concentrated cells from the final product bag.

Product features

• Designed to maximize recovery and minimize processing time.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: Chinese, English, French, German, Italian, and Spanish.

Kit / protocol compatibility matrix

Kit / protocol	CS-490.1	CS-470.1	CS-900.2	CS-430.1*
PeriCell	x	–	–	x
SmartRedux	x	–	–	–
ReadyCell	–	x	–	–
NeatCell	–	–	x	–
Adipose	–	–	x	–

*Maximum initial volume limited to 600 mL.

Ordering information

PeriCell: Product #14203

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.

 Download PDFProduct #14300

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

NeatCell is a protocol designed for isolation by means of a density gradient of the mononuclear cell (MNC) fraction from various cellular products into a small final volume of 8-20 mL. Alternatively, a fixed final volume of 45 mL can be chosen. In both cases, optimal granulocyte and Red Blood Cells (RBC) depletion is achieved.

Key features

NeatCell features
Initial volume	30 to 120 mL
Selectable final volume	8 to 20 mL, or 45 mL
Processing time	~1h15 minutes
MNC recovery	> 52%
RBC depletion	> 99%

Workflow

Step	Details
1	Prepare a kit with density gradient medium (via microbial filter), washing solution, and a syringe for collecting the final product (if applicable).
2	Connect initial product to the kit.
3	Install the kit on the Sepax.
4	The Sepax automatically performs the procedure including: • Priming • Isolation via a density gradient medium • Washing
5	Collect purified mononuclear cells from the final product bag or via a syringe.

Product features

• Automated procedure comprising of all required steps.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: Chinese, English, French, German, Italian, and Spanish.

Accessory for small final volume option

Use the separation chamber holder for the last steps of the procedure when collecting the final cells in a pre-connected syringe.

Kit / protocol compatibility matrix

Kit / protocol	CS-490.1	CS-470.1	CS-900.2	CS-430.1*
PeriCell	x	–	–	x
SmartRedux	x	–	–	–
ReadyCell	–	x	–	–
NeatCell	–	–	x	–
Adipose	–	–	x	–

*Maximum initial volume limited to 600 mL.

Ordering information

NeatCell: Product #14300

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.

 Download PDFProduct #14310

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

FamCord is a protocol designed for family blood bank and routine processing of umbilical cord blood (UCB) to isolate the nucleated cell component fraction enriched in hematopoietic stem cells. This protocol allows processing of initial volumes above 30 mL and below 220 mL (anti-coagulant included).

Key features

FamCord features
Initial volume	30 to 220 mL
Selectable final volume	15 to 25 mL
Processing time	20 to 40 minutes
Average TNC recovery	> 80% (dependent on initial volume)

Workflow

Step	Details
1	Prepare a kit with the initial product.
2	Install the kit on the Sepax.
3	The Sepax automatically performs the procedure.
3	Collect the final volume of nucleated cell fraction.

Product features

• For mono stopcock (reduced) kits.
• User selectable sedimentation profiles.
• Traceability: possibility to use the traceability kit of the Sepax (barcode reader + printer) in order to trace specific identification criteria and print a report at the end of each procedure.
• Simplified user selectable parameters.
• User interface available in English, French, German, Spanish, and Italian.

Kit / protocol compatibility matrix

Kit / protocol	CS-570.4	CS-570.1m	CS-570.4mb	CS-570.3m	CS-430.1	CS-470.0
FamCord	x	x	x	x	–	–
PlasmaFree	–	–	–	–	x	x

Ordering information

FamCord: Product #14310

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.

 Download PDFProduct #14308

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

The Dilution protocol is designed for diluting cell products and splitting cells into multiple bags with homogeneous concentrations.

Key features

Dilution features
Dilution volume	220 mL
Bag splits	Up to five
Average TNC recovery	> 90% (splitting) 100% (dilution)
Cell concentration variability	< 10% (splitting)

Workflow

Step	Details
1	Prepare a washing solution (dilution).
2	Connect the initial product to a kit.
3	Install the kit on the Sepax.
4	The Sepax automatically performs the selected procedure: • Dilution of final bag, or • Splitting of initial bag into multiple final bags.

Product features

• Interactive interface for active user guidance.
• Main user interface available in: English, French, German, Italian, and Spanish.

Kit / protocol compatibility matrix

Kit / protocol	CS-600.1	CS-900.2	CS-490.1
CordWash	x	–	–
SmartWash	x	–	–
CultureWash	x	x	–
Dilution	x	x	x

Ordering information

Dilution: Product #14308

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.

 Download PDFProduct #14309

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

CultureWash is designed for washing, exchanging medium, and concentrating cells. It offers flexible parameters and small final volume capabilities when manufacturing cellular therapy products.

Key features

Features	CultureWash
Initial volume	30 to 880 mL
Selectable final volume	8 to 220 mL*
Average CD34+ recovery	>80%
Average CD34+ viability	95%

*If product volume reduced to less than 20 mL, CS-900.2 single-use kit and Separation Chamber Holder are needed.

Workflow

Step	Details
1	Prepare a washing solution.
2	Prepare a kit with the initial product.
3	Install the kit on the Sepax.
4	The Sepax automatically performs the procedure including: • Primary dilution • Osmolarity balancing • Sedimentation and initial product rinsing • Supernatant extraction • Cell resuspension • Cell extraction into a final product bag.

Product features

• Dilution ratio: Defines the volume of washing solution used to dilute the initial product for osmolarity balancing.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: English, French, German, Italian, and Spanish.

Kit / protocol compatibility matrix

Kit / protocol	CS-600.1	CS-900.2**	CS-490.1
CordWash	x	–	–
SmartWash	x	–	–
CultureWash	x	x	–
Dilution	x	x	x

**The CS-900.2 kit is only validated for initial volumes up to 220 mL. Higher volumes must be validated by the user.

Ordering information

CultureWash: Product #14309

Separation Chamber Holder: Product #6033

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.